Journal: Nature Communications

Article Title: 3D extracellular matrix microenvironment in bioengineered tissue models of primary pediatric and adult brain tumors

doi: 10.1038/s41467-019-12420-1

Figure Lengend Snippet: Cytokine and MMP/TIMP media profiling from 3D bioengineered cultures. a MMP/TIMP release profile (reported in pg mL −1 ) of 3D ependymoma cultures infused with CLG1-based hydrogels. n = 5 pooled samples per condition. b MMP/TIMP release profile (reported in pg mL −1 ) of 3D GBM cultures infused with CLG1-based hydrogels alongside 3D GBM constructs with no hydrogel added. n = 5 pooled samples per condition. c Cytokine release profile at 1 mo of 3D GBM cultures infused with CLG1-based hydrogels (reported as fold change over control media). n = 7 pooled samples per condition. d Cytokine release profile at 6.5 mo of 3D GBM cultures infused with HA-based hydrogels at 5.5 mo time point (reported as fold change over control media). n = 5 pooled samples per condition. Supplementary Table lists the full forms of the cytokine abbreviations. Source data are provided as a Source Data file

Article Snippet: Multiplex Quantibody matrix metalloproteinases/tissue inhibitors of matrix metalloproteinases (MMP/TIMP) and cytokine arrays (RayBiotech) were used to semi-quantitatively or qualitatively compare the proteases/protease inhibitors and cytokines released by the tumor cells cultured in the 3D bioengineered brain tumor model with different ECM environments.

Techniques: Construct

Journal: Experimental neurology

Article Title: Scavenging reactive oxygen species inhibits status epilepticus-induced neuroinflammation

doi: 10.1016/j.expneurol.2017.08.009

Figure Lengend Snippet: Following an inciting injury i.e. status epilepticus (SE), there is increased production of reactive oxygen species (ROS), which can in turn activate microglial cells resulting in pro-inflammatory cytokine production. Cytokine production can result in further activation of the microglia and ROS production. Inflammatory cytokines can activate downstream receptors, leading to activation of certain kinases, which can result in network reorganization, neuronal injury and eventual development of epilepsy. i.e. epileptogenesis. Blocking ROS production by a catalytic antioxidant results in attenuation of microglial activation and inflammatory cytokine production, potentially by attenuation of SE-induced activation of the redox-sensitive mTOR pathway.

Article Snippet: Levels of the pro-inflammatory cytokines, TNF-α, IL-1β, IL-6 and KC/GRO (keratinocyte chemoattractant/growth-related oncogene), were measured using a rat multiplex pro-inflammatory cytokine array from Mesoscale Discovery (MSD) according to manufacturer’s instructions.

Techniques: Activation Assay, Blocking Assay